期刊论文

Cloning and bioinformatics analysis of LiUFGT gene of Lagerstroemia indica

中文

湖南林业科技

1003-5710

2023

50

01

24-30

10.3969/j.issn.1003-5710.2023.01.004

长沙市杰出创新青年培养计划(kq2106090)。

本校为第一机构

为探究类黄酮葡萄糖基转移酶基因(UFGT)在紫薇花青素生物合成中的分子机制及表达情况,本文以紫薇花瓣为试材,根据紫薇转录组序列设计特异性引物,通过PCR克隆LiUFGT基因开放序列,进行生物信息学分析,再对LiUFGT基...展开更多 为探究类黄酮葡萄糖基转移酶基因(UFGT)在紫薇花青素生物合成中的分子机制及表达情况,本文以紫薇花瓣为试材,根据紫薇转录组序列设计特异性引物,通过PCR克隆LiUFGT基因开放序列,进行生物信息学分析,再对LiUFGT基因进行荧光定量PCR分析。结果表明:LiUFGT基因的开放阅读框长1 431 bp,编码476个氨基酸,相对分子量为52 465.22 Da,理论等电点为5.66;LiUFGT蛋白为不稳定疏水蛋白,...

To explore the molecular mechanism and expression of flavonoid glucosyltransferase gene(UFGT) in anthocyanin biosynthesis of Lagerstroemia indica, the petals of Lagerstroemia indica were sellected as materials, and specific primers were designed according to the transcr...MORE To explore the molecular mechanism and expression of flavonoid glucosyltransferase gene(UFGT) in anthocyanin biosynthesis of Lagerstroemia indica, the petals of Lagerstroemia indica were sellected as materials, and specific primers were designed according to the transcrip...