期刊论文

中文

湖南林业科技

1003-5710

2021

48

02

55-60

10.3969/j.issn.1003—5710.2021.02.010

湖南省自然科学基金青年基金（2019JJ50305）； 湖南省林业科技创新专项（XLK201912）；

本校为第一机构

以"紫韵"紫薇为实验材料,利用cDNA末端快速克隆技术得到紫薇的F3H（LiF3H）基因的全长序列,进行生物信息学分析。结果表明:LiF3H基因的cDNA序列为1372 bp,最大开放阅读框为1116 bp,编码371个蛋白质;其蛋白分子量为41.600 4 KD,等电点为5.26,含依赖Fe2+和2—酮戊二酸的双氧酶超家族结构域,不含信号肽和跨膜结构域,该蛋白是不稳定的亲水性蛋白。系统进化树显示,LiF3H蛋白与石榴F3H蛋白的亲缘关系最近。

The full-length sequence of F3H gene(LiF3H)of Lagerstroemia indica‘Ziyun’was obtained by cDNA terminal rapid cloning technique,and bioinformatics analysis was performed.The results sho wed that the LiF3H gene had a cDNA sequence of 1372 bp,with a maximum open reading frame of 1116 bp,and encoded 371 proteins.The molecular weight of the protein was 41.6004 KD,while the isoelectric point was 5.26.It contains the Fe2+and 2-ketoglutarate dependent dioxygenase superfamily domain,but didn t contain the signal peptide and the transmembrane domain.Th...