Ulcerative colitis is a major inflammatory bowel disease (IBD), characterized by inflammation within the gastrointestinal tract through chronic or relapsing immune system activation. The aim of this study is to investigate the potential protective effect of oat beta-glucan (beta G) against colitis induced by DSS in mice. Eighty mice were randomly divided into the control group (no DSS, no beta G), DSS group (DSS only), DSS + L-beta G group (DSS plus 500 mg per kg beta G), and DSS + H-beta G group (DSS plus 1000 mg per kg beta G). Compared with the DSS group, administration of beta G significantly reduced clinical symptoms with less weight loss, diarrhea and shortening of the colon, the severity of colitis was significantly inhibited as evidenced by the reduced disease activity index (DAI) and degree of histological damage in colon. Moreover, treatment with beta G not only decreased myeloperoxidase activity (MPO), and nitric oxide (NO) and malondialdehyde (MDA) levels, but also inhibited mRNA and protein expression of pro-inflammatory factors such as TNF-alpha, IL-1 beta, IL-6 and iNOS. This suggests that oat beta G in diet might exhibit an anti-inflammatory function against colitis through inhibition of expression of pro-inflammatory factors.
本发明公开了一种发酵制备米渣蛋白抗氧化肽的方法，包括如下步骤：（1）利用霉菌对米渣进行固态发酵：在米渣中加入水，米渣和水的重量比为（15‑25）:9，搅拌浸润，灭菌后接种霉菌于30‑35℃条件下进行前期发酵2‑4天，得成曲；然后于40‑42℃条件下进行后期发酵4‑6天，得发酵产物；（2）将发酵产物通过超滤膜、凝胶层析过滤、制备型RP‑HPLC进行抗氧化产物的分离纯化；即得米渣蛋白抗氧化肽F2d和F2e，所述F2d序列如SEQ ID NO.1所示，所述F2e序列如SEQ ID NO.2所示。利用霉菌固态发酵米渣制取抗氧化产物，可极大降低米渣综合利用的生产成本，丰富了米渣作为食品原料的应用范围，提高了米渣综合利用的技术水平和商品价值，并且为天然抗氧化产物的研究开辟了新途径。