The expression analysis of genes in fatty acid biosynthesis pathway during the seed development of tung tree
作者:
Jiang, Guixiong;Chen, Hao;Tan, Xiaofeng
期刊:
International Journal BIOautomation ,2013年17(2):73-82 ISSN:1314-1902
通讯作者:
Tan, X.(tanxiaofengcn@126.com)
作者机构:
[Chen, Hao; Jiang, Guixiong; Tan, Xiaofeng] The Key Lab of Non-wood Forest Products of State Forestry Administration, College of Forestry, Central South University of Forestry and Technology, Changsha 410004, Hunan, China;[Jiang, Guixiong] Forestry Department of Guangxi Zhuang Autonomous Region, Nanning 530022, Guangxi, China
通讯机构:
[Tan, X.] T;The Key Lab of Non-wood Forest Products of State Forestry Administration, College of Forestry, Central South University of Forestry and Technology, China
关键词:
Differentially expressed genes;Expression profiles;Fatty acid biosynthesis;Seed development;Transcriptome;Tung tree
摘要:
Tung tree (Vernicia fordii) is one of the important oil plants in China. The current researches on tung tree are mainly focused on the cultivation and breeding while the molecular mechanisms hidden in the back of tung tree's phenotypes are still uncovered. This research compared the transcriptome of three different stages during tung tree's seed development using RNA-Seq and obtained a lot of differentially expressed Unigenes. Through GO classification and pathway enrichment analysis, all of these differentially expressed Unigenes were classified into 128 signaling pathways including fatty acid biosynthesis. Fourteen homologous proteins were obtained when the sequences of 54 Unigenes within fatty acid biosynthesis pathway were aligned against KEGG database and the expression profiles of the genes encoding these proteins during seed development were analyzed. This research provides necessary data platform to elucidate the molecular mechanism underlies fatty acid biosynthesis of tung tree's seeds and theoretical guidance of tung tree varieties' improvement to increase output of tung oil.
语种:
英文
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油桐ACPase基因的克隆及生物信息学分析
作者:
周俊琴;谭晓风;袁军;刘美兰
作者机构:
中南林业科技大学 经济林培育与保护教育部重点实验室,湖南 长沙 410004
会议名称:
第三届中国林业学术大会
会议时间:
2013-09-01
会议地点:
福州
会议论文集名称:
第三届中国林业学术大会论文集
关键词:
油桐;酸性磷酸酶;基因克隆;生物信息学
摘要:
根据油桐转录组酸性磷酸酶蛋白全长序列设计引物,通过RT-PCR直接扩增的方法从油桐种子中克隆到了酸性磷酸酶基因.对该cDNA序列、核苷酸序列的相似性、理化性质、疏水性、二级结构、三级结构及是否跨膜进行了分析预测,进行了多序列比对并构建了系统树.结果表明:油桐ACPase开放阅读框为1152bp,编码384个氨基酸残基,相对分子质量为40.94kDa,理论PI为5.30,属可溶性蛋白.二级结构主要由不规则卷曲、α-螺旋和β-折叠构成.同源性和进化树的预测分析显示,油桐ACPase蛋白与蓖麻的酸性磷酸酶蛋白同源性达到了78.9%,两者均属大戟科.该结果为进一步分析油桐ACPase的功能奠定了基础。
语种:
中文
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油茶果糖-1, 6-二磷酸醛缩酶基因(CoFBA4)的分子特征与表达分析
作者:
曾艳玲;谭晓风;蒋瑶;刘敏;王建勇;...
期刊:
林业科学 ,2013年49(11):164-170 ISSN:1001-7488
作者机构:
[曾艳玲; 谭晓风; 蒋瑶; 刘敏; 王建勇; 周俊琴] 中南林业科技大学
关键词:
油茶;果糖-1;6-二磷酸醛缩酶;基因克隆;基因表达;亚细胞定位
摘要:
果糖- 1,6 - 二磷酸醛缩酶( fructose-1,6-diphosphate aldolase,FBA,EC4. 1. 2. 13 ),简称醛缩酶,是糖酵解代谢途径中第4步关键酶,催化果糖-1,6 - 二磷酸( Fru-1,6-BP)可逆地裂解为磷酸二羟丙酮( DHAP)和3 - 磷酸甘油醛( G-3-P) ( Rutter,1964)。其中,DHAP 经甘油磷酸脱氢酶的催化,还原生成3 - 磷酸甘油,然后在脂酰转移酶的作用下生成磷脂酸,再经磷脂酸磷酸酶水解,形成油脂合成必需的酯化甘油骨架(Vigeolas et al.,2007); G-3-P则经过多步反应最终形成丙酮酸,产生乙酰辅酶A,经乙酰辅酶A 羧化酶作用形成脂肪酸合成的底物丙二酸单酰辅酶A,经过一系列聚合反应形成油脂合成的另一必需元素脂肪酸( Thelen et al.,2002)
语种:
中文
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杨树MIR169基因家族分子进化分析
作者:
刘志祥;曾超珍;谭晓风
期刊:
遗传 ,2013年35(11):1307-1316 ISSN:0253-9772
通讯作者:
Liu, Z.X.
作者机构:
[刘志祥] College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China. liuzx@csuft.edu.cn;[Zeng, Chao-Zhen] College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China;[谭晓风] College of Forestry, Central South University ofForestry and Technology, Changsha 410004, China
关键词:
毛果杨;MIR169基因家族;分子进化
摘要:
MicroRNA(miRNA)是真核生物中普遍存在的一类参与基因表达调控的小分子RNA。ptc-MIR169基因家族有33个成员, 是杨树中规模最大的miRNA基因家族。研究MIR169基因家族的进化对揭示杨树miRNA基因的进化机制具有重要的意义。文章对毛果杨MIR169基因家族的分子系统发育、基因倍增模式、表达分化和靶基因进行了分析。结果表明, 染色体大片段重复和串联重复在毛果杨MIR169基因家族扩张中均具有重要作用; MIR169基因家族在表达方式上已经出现了较大的分化; MIR169基因家族可能在杨树中已经形成了复杂的调控网络, 对杨树的生长发育和适应性等具有重要的调控作用。文章为杨树及杨柳科相关植物中miRNA基因家族的分子进化研究提供了参考。
语种:
中文
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油桐β-酮脂酰-ACP还原酶(KAR)基因的克隆与序列分析
作者:
刘美兰;谭晓风;龙洪旭;张琳;周俊琴;...
期刊:
江西农业大学学报 ,2013年35(5):962-969 ISSN:1000-2286
作者机构:
[刘美兰; 周俊琴; 龙洪旭; 王建勇; 张琳; 谭晓风] 中南林业科技大学
关键词:
油桐;β-酮脂酰-ACP还原酶;生物信息学
摘要:
以葡萄桐近成熟种子为材料,根据油桐转录组测序分析结果设计引物,利用RT-PCR技术克隆获得了2条KAR基因的全长cDNA,分别命名为Vf_KAR1和Vf _KAR2(GenBank登录号:KF025646,KF025647).生物信息学分析结果表明,Vf_KAR1和Vf_KAR2基因的CDS长度分别为993 bp和900 bp,分别编码331个和300个氨基酸;与其它物种的KAR氨基酸序列具有较高的相似性,Vf_KAR1与蓖麻的相似度最高,达82%;Vf_KAR2基因与花生、辣椒的相似度高达76%;2条KAR基因的多肽都含叶绿体转运肽,但是都没有信号肽;Vf_KAR1有1个明显的跨膜螺旋拓扑结构和1个疑似跨膜螺旋拓扑结构,Vf_KAR2有2个疑似的跨膜螺旋拓扑结构;肽链都表现为疏水性,都有典型的活化中心三联体(Ser-Tyr-Lys);三级结构都有典型的Rossmarn折叠特征.因此,初步推测Vf_KAR1和Vf_KAR2是油桐β-酮脂酰-ACP还原酶KAR基因家族的两个成员.
语种:
中文
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Effect of Phosphates on the Growth, Photosynthesis, and P Content of Oil Tea in Acidic Red Soils
作者:
Yuan, Jun;Tan, Xiaofeng;Yuan, Deyi;Zhang, Xuejie;Ye, Sicheng;...
期刊:
Journal of Sustainable Forestry ,2013年32(6):594-604 ISSN:1054-9811
通讯作者:
Yuan, J.(332549355@qq.com)
作者机构:
[Yuan, Jun; Tan, Xiaofeng] The Graduate School, Beijing Forestry University, Beijing, China;[Zhang, Xuejie; Zhou, Junqin; Ye, Sicheng; Yuan, Deyi] The Key Lab of Non-Wood Forest Nurturing and Protection of the National Ministry of Education, Central South University of Forestry and Technology University, Changsha, Hunan, China
通讯机构:
[Jun Yuan] T;The Graduate School, Beijing Forestry University, 35 Qinghuadonglu, China
关键词:
Camellia oleifera;acidic red soil;chlorophyll a fluorescence;organic phosphates;sparingly soluble phosphates
摘要:
Phosphorus (P) is a limiting factor in Camelia oleifera production as P is easily fixed by iron and aluminium oxides in acidic red soils. To better understand sparingly soluble and organic P utilization by oil tea in these soils, the growth and physiological responses of C. oleifera seedlings and P contents in soil and plants were investigated under Al-P, Ca-P, Fe-P, and O-P treatments. Plant growth was increased significantly when Ca-P was added, followed by Al-P and Fe-P. Net photosynthetic rate (Pn) was increased for all P-treated seedlings, and P treatments except for Fe-P also increased SPAD values. Ca-P, Al-P, and Fe-P treatments increased Fv/Fm, Fv'/Fm', yield, and qP (photochemical quenching). Ca-P and Al-P increased available P the most, while Fe-P increased TP the most in both rhizosphere and non-rhizosphere soils. Available P level in rhizosphere soil was significantly higher than that in non-rhizosphere soil. P treatments significantly increased P content in all plant samples. These results demonstrated that Al-P and Ca-P can be effectively utilized by oil tea seedlings in acidic red soils, suggesting that sparingly soluble inorganic P is the main source of plant P in unfertilized soil. ©2013 Copyright Taylor and Francis Group, LLC.
语种:
英文
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桉叶不饱和脂肪酸的提取研究及成分分析
作者:
杨巧丽;谢耀坚;谭晓风;张婧;黄赤夫;...
期刊:
林产化学与工业 ,2013年33(2):113-118 ISSN:0253-2417
通讯作者:
Wu, Z.-H.(wzhua2889@163.com)
作者机构:
[张婧; 吴志华; 杨巧丽; 谢耀坚] Eucalypt Research Centre, Zhanjiang 524022, China;[黄赤夫] College of Dentistry, Medicine Center, University of Kentucky, Lexington 40503, United States;[杨巧丽; 谭晓风] Key Lab. of Non-wood Forest Products of State Forestry Administration, Central University of Forestry and Technology, Changsha 410004, China
通讯机构:
Eucalypt Research Centre, China
会议名称:
第十届中国林业青年学术论坛
会议时间:
2012-09-01
会议地点:
南京
会议论文集名称:
第十届中国林业青年学术论坛论文集
关键词:
桉叶;不饱和脂肪酸;正交试验;提取;成分分析
摘要:
用不同的溶剂、物料粒度、料液比、浸泡时间及萃取时间对桉叶不饱和脂肪酸进行提取的正交试验,筛选出最优的提取方案并对提取物甲酯化处理后通过GC-MS分析其成分。在进行了L_(16)(4~5)正交试验之后,研究得到当溶剂为丙酮、物料粒度为0.075~0.15mm、料液比为1∶15(g∶mL)、浸泡时间为8h及超声波处理1.5h时提取得率为14.9%,GC-MS分析,鉴定出6个化合物,其中有5个脂肪酸类化合物,如不饱和脂肪酸亚油酸和亚麻酸,10g桉叶中含有36.01 mg不饱和脂肪酸。桉叶中含有重要的不饱和脂肪酸,具有开发利用价值。
语种:
中文
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不同磷源对酸性红壤养分及油茶幼苗生长的影响
作者:
袁军;谭晓风;叶思诚;周乃富
期刊:
西北农林科技大学学报(自然科学版) ,2013年41(4):155-160 ISSN:1671-9387
作者机构:
[袁军] 北京林业大学研究生院;[谭晓风; 叶思诚; 周乃富] 中南林业科技大学
关键词:
油茶;难溶磷;酸性红壤;营养;生长
摘要:
[目的]研究不同磷源对酸性红壤养分及油茶生长的影响,为油茶施肥提供参考。[方法]以油茶'湘林4号'为试材,以不施外源磷为对照,通过盆栽试验,研究无机难溶磷(Ca-P、Al-P和Fe-P)和有机磷源(O-P)对酸性红壤养分、油茶幼苗生长及树体营养水平的影响。[结果]不同磷源对酸性红壤养分含量的影响有差异,其中Fe-P显著提高土壤全磷含量,而Ca-P和Al-P则显著提高土壤有效磷含量;在根际土壤中,Ca-P和Al-P处理的有效磷含量较对照分别提高120倍和125倍。油茶根际与非根际土壤中的养分含量具有明显差异。Ca-P和Al-P明显提高了油茶苗高、地上部干质量和根系鲜质量,降低根冠比,而Fe-P和O-P的作用不明显。在土壤中添加不同磷源显著提高了油茶老叶、新叶以及根系中的磷含量,并明显影响植株中的氮和钾含量。[结论]无机难溶磷和有机磷可提升酸性红壤肥力并促进油茶生长,其中以Ca-P和Al-P作用最明显;对于长期不施肥的油茶林地,Al-P和其他难溶磷能被油茶有效利用。
语种:
中文
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油桐叶片愈伤组织诱导及植株再生
作者:
谭晓风;李泽;张琳;龙洪旭;袁军;...
期刊:
植物生理与分子生物学学报 ,2013年49(11):1245-1249 ISSN:1671-3877
通讯作者:
Tan, X.-F.
作者机构:
[谭晓风; 李泽; 张琳; 龙洪旭; 袁军; 曾艳玲; 林青] 中南林业科技大学
通讯机构:
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, China
关键词:
油桐;愈伤组织;不定芽;植株再生
摘要:
以油桐无菌苗叶片为试材, 研究不同种类及浓度的植物生长调节剂对愈伤组织诱导、分化、增殖及生根的影响。结果表明: 叶片愈伤组织的最佳诱导培养基为1/2MS+2.0 mg·L~(-1) 6-BA+1.0 mg·L~(-1) 2,4-D, 诱导率达100%; 最佳分化培养基为1/2MS+3.0 mg·L~(-1) 6-BA+0.1 mg·L~(-1) IBA+0.05 mg·L~(-1) IAA, 分化率为86.36%; 最佳继代增殖培养基为MS+3.0 mg·L~(-1) 6-BA+0.05mg·L~(-1) IBA; 最佳生根培养基为1/2MS+0.1 mg·L~(-1) IBA, 生根率93.83%。炼苗后移栽到泥炭土:珍珠岩:蛭石=2:1:1的基质中, 成活率达92%以上。
语种:
中文
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A new member of PAL family initiating secondary metabolism during fatty acid biosynthesis in Camellia oleifera seeds
作者:
Xiaofeng Tan;Hongpeng Chen;Dangquan Zhang;Fangming Hu
期刊:
Advanced Science Letters ,2012年10(1):191-195 ISSN:1936-6612
通讯作者:
Tan, X.
作者机构:
Key lab of Non-wood Forest Product of State Forestry Administration, Changsha 410004,China;Central South University of Forestry and Technology, Changsha 410004, China;National Biotechnology Lab of Camellia oleifera Science Center, Changsha 410004, China;National Biotechnology Lab of Camellia oleifera Sci;[Tan X.; Zhang D.; Hu F.] The Key Lab of Non-Wood Forest Product of Forestry Ministry, Changsha 410004, China, College of Forestry, Central South University of Forestry and Technology, Changsha 410004, China, China The National Biotechnology Lab oflCamellia oleifera Science Center, Changsha 410004, China
通讯机构:
[Tan, X.] T;The Key Lab of Non-Wood Forest Product of Forestry Ministry, Changsha 410004, China
会议名称:
2009第四届国际基因组学大会)
会议时间:
2009-11-3
会议地点:
深圳
会议主办单位:
深圳华大基因研究院
会议论文集名称:
2009第四届国际基因组学大会)论文集
关键词:
Camellia oleifera;Fatty acid biosynthesis;Phenylalanine ammonialyase;Seeds
摘要:
In the process of monolignol biosynthesis,phenylalanine ammonialyase (PAL) is thought to be the first step towards monolignol biosynthesis and other phenolic secondary metabolisms derived from primary metabolism, and plays multiple important roles involved many biosynthesis processes of flavone, lignin and alkaloid to defense diseases and stress. Tea-oil, extracted from seeds of Camellia oleifera which only grows in South of China, has been famous all over the world for its nutrient constituents including oleic acid, linoleic acid and other unsaturated fatty acids. However, the fatty acid biosynthesis could be achieved successfully in Camellia oleifera seeds though the trees grow in the environment of diseases and insect damage, high temperature and drought with the contribution of lax on leaf surface and saponin in the seed out shell. The analysis of the components of the out shell of the seeds suggested that the content of saponin was up to 34.9%. In order to reveal the relationship between fatty acid and saponin biosynthesis stimulated by the stress of outside growth environment, a novel PAL gene was obtained and named as Co-PAL in the research. Bioinformatics analysis showed that the full-length of Co-PAL cDNA was 2426 bp in length, including a 2118 bp ORF encoding 706 amino acids, a 159 bp 5′ UTR and 174 bp 3′ UTR with a polyA tail. Multiple sequences alignment displayed that the deduced protein shared 8% similarity with defined PAL from Camellia sinensis And the protein characters performed by Antheprot 5.0 showed the pI was 6.115, it could be dissolved in water for its hydrophilicity, the molecular weight was about 77.07 kD, a potential cleavage of signal peptide was found at Ala 281, and three transmembrane regions distributed at N-terminal. In addition, many sites essential for PAL activities conserved indicated Co-PAL was a new member of PAL family which was also found in different plant species. © 2012 American Scientific Publishers. All rights reserved.
语种:
英文
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油茶高亲和磷转运蛋白基因Pht1;2的克隆及生物信息学分析
作者:
周俊琴;谭晓风;袁军;龙洪旭;叶思诚;...
作者机构:
中南林业科技大学 经济林培育与保护教育部重点实验室,长沙 410004;北京林业大学,北京 100083
会议名称:
中国林学会经济林分会2012年学术年会
会议时间:
2012-11-01
会议地点:
浙江临安
会议论文集名称:
中国林学会经济林分会2012年学术年会论文集
关键词:
油茶;磷转运子;生物信息学
摘要:
以油茶品种‘ 华硕’为试材,通过 RT-PCR 和 RACE 的方法克隆出油茶磷酸转运子Pht1 基因家族一个成员的全长cDNA序列,命名为CoPht1;2(GenBank 登录号:JX412956.1),通过生物信息学技术对其序列的理化性质、结构与功能进行分析和预测.结果表明:CoPht1;2 CDS 长度为1 590 bp,编码530个氨基酸,与其它物种的Pht1 氨基酸序列具有较高的相似性,其中与杨柳科毛果杨的 Pht1相似性最高,达到 77.5%;该基因所编码蛋白质的分子量为58.02 kD,理论等电点pI 为8.97,二级结构主要由α-螺旋、β-折叠和不规则卷曲构成,包含12个明显的跨膜螺旋拓扑结构;预测显示该蛋白是一个疏水跨膜蛋白,具有磷转运蛋白的主要特征,初步判定其与油茶磷吸收有关,其功能有待进一步验证.
语种:
中文
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油桐种子FAD2基因全长cDNA序列分析
作者:
谢禄山;谭晓风;张琳;龙洪旭
期刊:
经济林研究 ,2012年30(2):1-9 ISSN:1003-8981
作者机构:
中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙410004;中南林业科技大学风景园林学院,湖南长沙410004;中南林业科技大学林学院,湖南长沙410004;中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙,410004;[龙洪旭; 谢禄山; 张琳; 谭晓风] 中南林业科技大学
关键词:
油桐;种子;cDNA文库
摘要:
油桐种子中FAD2催化形成的亚油酸是合成桐油酸的直接原料,研究油桐种子中的FAD2基因对提高桐油酸的产量具有实际意义。将油桐对年桐近成熟种子cDNA文库中的16个FAD2克隆子进行CAP3拼接,再进行BLAST比对,并进行DNAMAN分析,结果表明所克隆的基因序列为FAD2全长cDNA序列,其长度为1 537 bp,含有1个完整的编码序列,长度为1 146 bp(106~1 255 bp),编码383个氨基酸。酶蛋白相对分子质量44 144.4 u,等电点为8.57,氨基酸序列N端有6个残基的信号肽序列,有5个跨膜结构域,N端、C端及中间各有一段表现为强亲水性,酶活性中心为3个保守的组氨酸簇。在系统进化上,油桐FAD2基因与千年桐的亲缘最近,与蓖麻、乌桕、橡胶树、麻疯树等大戟科植物的亲缘关系较近,与油橄榄、花生、芝麻等植物的亲缘较远,与油茶的亲缘关系更远。
语种:
中文
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油桐β-酮脂酰-ACP合酶Ⅲ基因克隆及其序列分析
作者:
严方方;谭晓风;龙洪旭
作者机构:
中南林业科技大学 经济林培育与保护教育部重点实验室,长沙 410004;中南林业科技大学 经济林育种与栽培国家林业局重点实验室 长沙 410004
会议名称:
中国林学会经济林分会2012年学术年会
会议时间:
2012-11-01
会议地点:
浙江临安
会议论文集名称:
中国林学会经济林分会2012年学术年会论文集
关键词:
油桐;基因克隆;序列分析:结构预测
摘要:
β-酮脂酰-ACP合酶-KAS是脂肪酸合成中的一种缩合酶.本研究以近成熟的油桐种子为材料,利用改良CTAB法提取总RNA,并根据GenBank 中蓖麻等已知的β-酮脂酰-ACP合酶基因KASⅢ 的序列设计引物,利用简并PCR、RACE 等技术克隆得到油桐种子中KASⅢ基因全长cDNA.该基因序列全长1901bp,开放阅读框为1209bp,编码403个氨基酸,通过与其他物种的KASⅢ氨基酸序列比对,油桐KASⅢ基因与与麻疯树的同源性最高,达90%.软件预测表明,KASⅢ蛋白等电点为5.78,存在跨膜结构域,整个蛋白质基本上是表现出亲水性的.
语种:
中文
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Synthesis of tung oil anhydride-ester polyol
作者:
Du, Xunjun* ;Sun, Hanzhou;Wu, Yuxiong;Zhou, Jinhua;Tan, Xiaofeng;...
期刊:
Advanced Materials Research ,2012年418-420:693-697 ISSN:1022-6680
通讯作者:
Du, Xunjun
作者机构:
[Du, Xunjun; Zhou, Jinhua; Wu, Yuxiong; Sun, Hanzhou] Cent South Univ Forestry & Technol, Inst Appl Chem, Changsha 410004, Hunan, Peoples R China.;[Tan, Xiaofeng; Zhang, Dangquan] Cent South Univ Forest & Technol, Key Lab Non Wood Forest Prod State Forestry Adm, Changsha 410004, Peoples R China.;[Zhang, Huaiyun; Zhang, Dangquan] Cent South Unive Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.
通讯机构:
[Du, Xunjun] C;Cent South Univ Forestry & Technol, Inst Appl Chem, Changsha 410004, Hunan, Peoples R China.
会议名称:
2nd International Conference on Advances in Materials and Manufacturing Processes (ICAMMP 2011)
会议时间:
DEC 16-18, 2011
会议地点:
Guilin, PEOPLES R CHINA
会议主办单位:
[Du, Xunjun;Sun, Hanzhou;Wu, Yuxiong;Zhou, Jinhua] Cent South Univ Forestry & Technol, Inst Appl Chem, Changsha 410004, Hunan, Peoples R China.^[Tan, Xiaofeng;Zhang, Dangquan] Cent South Univ Forest & Technol, Key Lab Non Wood Forest Prod State Forestry Adm, Changsha 410004, Peoples R China.^[Zhang, Dangquan;Zhang, Huaiyun] Cent South Unive Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.
会议论文集名称:
Advanced Materials Research
关键词:
Ethylene glycol;Methyl-a-eleostearate- maleic anhydride adduct;Synthesis;Tung oil;Tung oil anhydride-ester polyol
摘要:
Tung oil is a special biomass resource in China. Tung Oil has the characteristic to be transformed into serial derivatives for its active Conjugated double bond. Especially, tung oil anhydride-ester polyol (TOAEP) can be used as substitute of polyester polyol and/or polyether polyol in the synthesis and modification of polyurethane materials. Through esterification and transesterification, methyl-α-eleostearate-maleic anhydride adduct (MEMAA), a derivative of tung oil, was reacted with ethylene glycol and tung oil anhydride-ester polyol (TOAEP) was obtained. The optimal preparation condition are as follows: p-toluene sufonic acid is used as the catalyst, mole ratio of ethylene glycol to MEMAA is 4:1, reaction temperature is 120°C ~130°C, reaction duration is 8 h. Under the optimal preparation conditions, the yield of TOAEP is up to 88.8% and the hydroxyl value is up to 318.0 mg KOH/g. The structure of TOAEP was confirmed by Infrared spectroscopy (IR).
语种:
英文
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Modification of polyurethane by Tung oil anhydride-ester polyol
作者:
Sun, Hanzhou* ;Du, Xunjun;Zhou, Jinhua;Xie, Lianwu;Wu, Yuxiong;...
期刊:
Advanced Materials Research ,2012年554-556:191-194 ISSN:1022-6680
通讯作者:
Sun, Hanzhou
作者机构:
[Sun, Hanzhou; Tan, Xiaofeng] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Hunan, Peoples R China.;[Du, Xunjun; Zhou, Jinhua; Wu, Yuxiong; Sun, Hanzhou; Xie, Lianwu] Cent S Univ Forestry & Technol, Appl Chem Inst, Changsha 410004, Peoples R China.;[Zhang, Huaiyun] Cent S Univ Forestry Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.
通讯机构:
[Sun, Hanzhou] C;Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Hunan, Peoples R China.
会议名称:
2nd International Conference on Chemical Engineering and Advanced Materials (CEAM 2012)
会议时间:
JUL 13-15, 2012
会议地点:
Guangzhou, PEOPLES R CHINA
会议主办单位:
[Sun, Hanzhou;Tan, Xiaofeng] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Hunan, Peoples R China.^[Sun, Hanzhou;Du, Xunjun;Zhou, Jinhua;Xie, Lianwu;Wu, Yuxiong] Cent S Univ Forestry & Technol, Appl Chem Inst, Changsha 410004, Peoples R China.^[Zhang, Huaiyun] Cent S Univ Forestry Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.
会议论文集名称:
Advanced Materials Research
关键词:
Modification;Polyurethane (PU);Tung Oil;Tung Oil Anhydride-Ester Polyol
摘要:
Polyurethane (PU), one of the six main categories of synthetic resins, has been used extensively in industry all over the world. Tung oil anhydride-ester polyol (TOAEP), prepared from the China special vegetable oil, Tung oil, was introduced into PU structure. The mechanical property, heat-resistance and morphological structure of the modified PU were studied. Experimental results showed that the hardness and tensile strength of the modified PU increases as the dose of TOAEP increasing while the elongation at break decreases, and the heat-resistance has been improved after modification. The modified PU's hard segment phase is compatible with the soft segment phase. ©(2012) Trans Tech Publications, Switzerland.
语种:
英文
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油茶分子育种研究
作者:
谭晓风
作者机构:
中南林业科技大学 经济林培育与保护教育部重点实验室,湖南长沙 410004;中南林业科技大学 经济林栽培与育种国家林业局重点实验室,湖南长沙 410004
会议名称:
中国林学会经济林分会2012年学术年会
会议时间:
2012-11-01
会议地点:
浙江临安
会议论文集名称:
中国林学会经济林分会2012年学术年会论文集
关键词:
油茶;分子育种;研究进展
摘要:
油茶分子育种是指按照人们的愿望,进行严密的设计,通过体外DNA重组技术和DNA转移技术,有目的地改造油茶的生物特性,以创造出新的品种类型的技术体系。油茶分子育种技术主要包括转基因育种和分子标记辅助选择育种。油茶分子标记辅助选择育种还处于分子标记开发阶段。转基因育种还处于前期的基础研究阶段,国内已经开展了油茶的EST、转录组、表达谱、基因注释、重要基因克隆及其功能研究,开展了部分重要基因对拟南芥、油菜等的转基因实验研究。油茶分子育种的主要目标是提高油茶种仁的含油率、改善茶油的品质、增强油茶的抗逆性能等方面。
语种:
中文
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油茶CoUXS1基因全长cDNA克隆及生物信息学分析
作者:
曾艳玲;谭晓风;王保明;龙洪旭;刘凯;...
期刊:
中国农学通报 ,2012年28(22):39-44 ISSN:1000-6850
作者机构:
[曾艳玲; 谭晓风; 王保明; 龙洪旭; 刘凯; 曾晓峰] 中南林业科技大学
关键词:
油茶;系统进化分析;结构域功能预测
摘要:
为了揭示油茶UDP-葡萄糖醛酸脱羧酶基因的序列特点和结构功能,为该基因的深入研究和开发应用奠定基础。根据油茶种子EST文库中调取的UDP-葡萄糖醛酸脱羧酶基因序列,设计特异引物,利用RT-PCR技术获得该基因全长cDNA克隆,并对其序列进行生物信息学分析。结果表明:该基因阅读框为1032bp,编码344个氨基酸,具有典型的UXS家族模体序列。与海岛棉GhUXS3基因亲缘关系最近,定位于细胞质,有较强的亲水性,将该基因命名为CoUXS1,GenBank登录号为JN017094。
语种:
中文
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油茶脂氢过氧化物裂解酶基因的克隆与序列分析
作者:
王建勇;谭晓风;龙洪旭;陈鸿鹏;刘凯;...
作者机构:
中南林业科技大学 经济林培育与保护教育部重点实验室 湖南长沙;中南林业科技大学 经济林栽培与育种国家林业局重点实验室,湖南长沙 410004
会议名称:
中国林学会经济林分会2012年学术年会
会议时间:
2012-11-01
会议地点:
浙江临安
会议论文集名称:
中国林学会经济林分会2012年学术年会论文集
关键词:
油茶;脂氢过氧化物裂解酶;基因克隆;序列分析
摘要:
以油茶近成熟种子为材料,根据油茶EST 文库中已知的脂氢过氧化物裂解酶EST 序列,利用3’RACE 与5’RACE 技术,克隆到脂氢过氧化物裂解酶的全长cDNA序列.该基因全长1648bp,,包含一个1476bp 开放阅读框长,编码491个氨基酸,5’与3’非编码分别为52bp、121bp.预测该蛋白相对分子量为54.7779KDa,等电点为6.77,是个叶绿体转运肽,N 端包含有22个疏水氨基酸残基组成的序列,不仅具有转运肽富含的丝氨酸,还含有大量转运肽中很少存在的脯氨酸.多序列比对发现油茶脂氢过氧化物裂解酶核苷酸序列与茶的相似性达到96%,因此将该基因命名co HPL.
语种:
中文
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油桐4个品种光合特性的日变化
作者:
李泽;谭晓风;袁军;龙洪旭;周乃富;...
作者机构:
中南林业科技大学 经济林培育与保护教育部重点实验室,湖南长沙 410004
会议名称:
中国林学会经济林分会2012年学术年会
会议时间:
2012-11-01
会议地点:
浙江临安
会议论文集名称:
中国林学会经济林分会2012年学术年会论文集
关键词:
油桐;净光合速率;光合特性;蒸腾速率;日变化
摘要:
利用Li-6400 便携式光合测定系统,测定了4个油桐品种光合指标的日变化。结果表明:(1)‘葡萄桐’、‘小米桐’、‘对年桐’的净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)日变化均呈单峰曲线,‘大米桐’的净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)的日变化呈双峰曲线;(2) 4个品种的胞间CO2 浓度(Ci)日变化呈不规则的“V”字型;(3) 净光合速率日变化平均值大小排序为:‘葡萄桐’>‘小米桐’>‘对年桐’>‘大米桐’;(4) 影响油桐净光合速率值的主要生态生理指标大小依次为:蒸腾速率>胞间CO2 浓度>光合有效辐射>气孔导度。
语种:
中文
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Synthesis of Tung oil-based bi-dihydrogen-maleimide
作者:
Zhou, Jinhua* ;Du, Xunjun;Sun, Hanzhou;Wu, Yuxiong;Tan, Xiaofeng;...
期刊:
Advanced Materials Research ,2012年554-556:787-791 ISSN:1022-6680
通讯作者:
Zhou, Jinhua
作者机构:
[Zhou, Jinhua; Du, Xunjun; Wu, Yuxiong; Sun, Hanzhou] Cent South Univ Forestry & Technol, Inst Appl Chem, Changsha 410004, Hunan, Peoples R China.;[Zhang, Huaiyun] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.;[Tan, Xiaofeng] Cent South Univ Forestry & Technol, State Forestry Adm, Key Lab Non Wood Forest Prod, Changsha 410004, Peoples R China.
通讯机构:
[Zhou, Jinhua] C;Cent South Univ Forestry & Technol, Inst Appl Chem, Changsha 410004, Hunan, Peoples R China.
会议名称:
2nd International Conference on Chemical Engineering and Advanced Materials (CEAM 2012)
会议时间:
JUL 13-15, 2012
会议地点:
Guangzhou, PEOPLES R CHINA
会议主办单位:
[Zhou, Jinhua;Du, Xunjun;Sun, Hanzhou;Wu, Yuxiong] Cent South Univ Forestry & Technol, Inst Appl Chem, Changsha 410004, Hunan, Peoples R China.^[Zhang, Huaiyun] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.^[Tan, Xiaofeng] Cent South Univ Forestry & Technol, State Forestry Adm, Key Lab Non Wood Forest Prod, Changsha 410004, Peoples R China.
会议论文集名称:
Advanced Materials Research
关键词:
Bismaleimide;Synthesis;Tung oil;Tung oil-based bi-dihydrogen-maleimide
摘要:
Started from Tung oil, a renewable biomass material of china, a new kind of bismaleimide, Tung oil-based bi-dihydrogen-maleimide (TOBBDHMI) was synthesized by the reaction of methyl-α-eleostearate-maleic anhydride adduct (MEMAA) and 4,4'-diphenylmethane disocyanate (MDI). The optimal conditions for synthesis were catalyst triethylamine dosage 1.0%, mole ratio of MEMAA to MDI 2.4:1, reaction temperature 75 °C and reaction time 2 h, which gave yield 82.4% for TOBBDHMI (calculated from MDI). The melting point of the product was 186.6 °C ~189.3 °C, initial decomposition temperature was 270 °C and final decomposition temperature was 510 °C, which showed the product has good heat resistance. The target product is potential to be used alone or with other bismaleimide monomers to improve polymeric materials' heat resistance and/or mechanical properties.©(2012) Trans Tech Publications, Switzerland.
语种:
英文
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