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Rapid evaluation of γ-aminobutyric acid in foodstuffs by direct real-time mass spectrometry

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成果类型:
期刊论文
作者:
WeiliLi;XiaoyuWu;XvYuan;WenhuaZhou;TaoWu
作者机构:
School of Food and Biotechnology, Xihua University, No. 9999 Hongguang Avenue, Chengdu 610039, People’s Republic of China
Key Laboratory of Processed Food for Special Medical Purpose, Central South University of Forestry and Technology, No. 498 Shaoshan Road, Changsha 410004, People’s Republic of China
语种:
中文
关键词:
DART;ESI;Gamma-aminobutyric acid;Germination;Highland barley;Bean
期刊:
Food Chemistry
ISSN:
0308-8146
年:
2019
卷:
277
页码:
617-623
机构署名:
本校为其他机构
摘要:
Direct analysis in real-time ionization coupled with mass spectrometry (DART–MS) was first applied for the rapid determination of gamma-aminobutyric acid (GABA) in foods. Samples of germinated barley and fermented beans containing GABA at different levels were used, and the results were compared with those obtained by ultrahigh-performance liquid chromatography coupled with electrospray ionization triple quadrupole mass spectrometry (UHPLC–ESI–MS). After a series of optimization, a simple sample extraction procedure using 30% methanol aqueous solution was conducted, followed by direct determination of sample extracts without chromatographic separation or prior derivatization. The optimized DART–MS method exhibited low limits of detection (0.040 mg·kg−1) and good recovery rates (88.6%–104%). The Aspergillus oryzae-fermented black beans produced the highest amount GABA. The results for the samples slightly varied between DART–MS and UHPLC–ESI–MS. Current findings indicate that DART–MS could be a high-throughput alternative to classic UHPLC–ESI–MS.

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