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A mitochondria targetable two-photon excited near-infrared fluorescent probe for imaging of H2O2 in live cells and tissues.

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成果类型:
期刊论文
作者:
Zhou, Liyi*;Ding, Haiyuan;Zhao, Wen;Hu, Shunqin
通讯作者:
Zhou, Liyi
作者机构:
[Zhou, Liyi; Zhao, Wen; Ding, Haiyuan] College of Food Science and Technology, Central South University of Forestry and Technology, Changsha 410004, PR China
[Zhou, Liyi; Hu, Shunqin] College of Life Sciences and Chemistry, Hunan University of Technology, Hunan 412007, PR China
[Zhou, Liyi] Hunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan 410004, PR China
[Zhou, Liyi] State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China. Electronic address: zhouly0817@163.com
通讯机构:
[Zhou, Liyi] State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China. Electronic address:
语种:
英文
关键词:
Hydrogen peroxide;Phenylboronic acid;Two-photon near-infrared;Mitochondrial image
期刊:
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
ISSN:
1386-1425
年:
2019
卷:
206
页码:
529-534
文献类别:
WOS:Article
所属学科:
WOS学科类别:Spectroscopy
入藏号:
WOS:000447570700067;PMID:30176429
基金类别:
National Natural Science Foundation of China [21605046]; Natural Science Foundation of Hunan Province [2017JJ3060]; Science and Technology Innovation Platform and Talent Project of Hunan Province [2017TP1021]
机构署名:
本校为第一且通讯机构
院系归属:
食品科学与工程学院
摘要:
Hydrogen peroxide (H2O2), a member of small-molecule reactive oxygen species (ROS), plays an important role in physiological and/or pathological process within live systems. Herein, to quantitatively investigate the biological role of H2O2 in subcellular level, we constructed of a novel two-photon (TP) in near-infrared (NIR) out fluorescent probe (TP-NIR-H2O2) for visualization of mitochondria H2O2 in living cells and tissues. Specifically, TPNIR-H2O2 utilized the oxonium ion as the mitochondrial targeting unit and phenylboric acid as the H2O2 reaction moiety. After the phenylboric acid moiety reaction with H2O2, TP-NIR-H2O2 displayed a similar to 105-fold fluorescence intensity enhancement in 665 nm. Selectivity experiment demonstrated that the probe can detect H2O2 with high selectivity over other ROS. Moreover, TP-NIR-H2O2 could be employed for imaging H2O2 in mice liver tissues with large tissue-image depth (50-170 mu m) under two-photon excitation (800 nm). (C) 2018 Elsevier B.V. All rights reserved.
参考文献:
Abo M, 2011, J AM CHEM SOC, V133, P10629, DOI 10.1021/ja203521e
Albers AE, 2006, J AM CHEM SOC, V128, P9640, DOI 10.1021/ja063308k
Ashki N, 2008, NEUROSCIENCE, V156, P107, DOI 10.1016/j.neuroscience.2008.06.050
Balaban RS, 2005, CELL, V120, P483, DOI 10.1016/j.cell.2005.02.001
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